B3GALTL Polyclonal antibody

B3GALTL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14601-1-AP

Synonyms

B3GALTL, B3Glc T, B3GLCT, B3GTL, Beta 1,3 glucosyltransferase, beta3Glc T, Gal T

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Validation Data Gallery

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  WB analysis of human heart using 14601-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14601-1-AP (B3GALTL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14601-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14601-1-AP (B3GALTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 14601-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14601-1-AP (B3GALTL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 14601-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14601-1-AP (B3GALTL antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14601-1-AP targets B3GALTL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GALTL fusion protein Ag6146
• Full Name: beta 1,3-galactosyltransferase-like
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC068595
• Gene Symbol: B3GALTL
• Gene ID (NCBI): 145173
• RRID: AB_2063186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.