Tested Applications
| Positive WB detected in | PC-12 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue, mouse spleen tissue, human thyroid cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19401-1-AP targets B3GAT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13550 Product name: Recombinant human CD57 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-334 aa of BC010466 Sequence: HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI Predict reactive species |
| Full Name | beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 45 kDa, 70 kDa |
| GenBank Accession Number | BC010466 |
| Gene Symbol | B3GAT1 |
| Gene ID (NCBI) | 27087 |
| RRID | AB_2878577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2W7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for B3GAT1 antibody 19401-1-AP | Download protocol |
| IHC protocol for B3GAT1 antibody 19401-1-AP | Download protocol |
| WB protocol for B3GAT1 antibody 19401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B3GAT1 Polyclonal antibody (catalog number 19401-1-AP) has 6 total citations. These appear in journals including Cell Death & Disease, Frontiers in Bioengineering and Biotechnology, Cancers (Basel), Gynecologic Oncology, BMC Medical Genomics, and Aging (Albany NY). The associated research fields span cancer biology and immunology, covering non-seminoma stemness and metastasis, cervical cancer, ovarian cancer, and glioblastoma, with a focus on immune infiltration, prognosis, and single-cell analyses. Most studies applied the antibody for IHC, with one using IF.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas | ||
Front Bioeng Biotechnol Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells. | ||
Cancers (Basel) The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer. | ||
Gynecol Oncol Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer. | ||
BMC Med Genomics Establishment and validation of an immune infiltration predictive model for ovarian cancer | ||
Aging (Albany NY) Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma |
