CD57 Polyclonal antibody

CD57 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19401-1-AP

Synonyms

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1



Tested Applications

Positive WB detected inPC-12 cells, SH-SY5Y cells
Positive IHC detected inmouse brain tissue, mouse spleen tissue, human thyroid cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19401-1-AP targets CD57 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD57 fusion protein Ag13550
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 45 kDa, 70 kDa
GenBank Accession NumberBC010466
Gene Symbol B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2878577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.

Protocols

Product Specific Protocols
WB protocol for CD57 antibody 19401-1-APDownload protocol
IHC protocol for CD57 antibody 19401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.

Authors - Sihui Yu
humanIHC

Gynecol Oncol

Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.

Authors - Jing Zhang
humanIF

Front Bioeng Biotechnol

Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.

Authors - Jie Chen
humanIHC

BMC Med Genomics

Establishment and validation of an immune infiltration predictive model for ovarian cancer

Authors - Zhenxia Song
human,mouseIHC

Aging (Albany NY)

Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma

Authors - Xiaonan Zhang