B3GAT1 Polyclonal antibody

B3GAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

5

Cat no : 19401-1-AP

Synonyms

GlcUAT-P, GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135

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Validation Data Gallery

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  WB analysis using 19401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19401-1-AP (B3GAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, mouse spleen tissue, human thyroid cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

19401-1-AP targets B3GAT1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GAT1 fusion protein Ag13550
• Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 45 kDa, 70 kDa
• GenBank Accession Number: BC010466
• Gene Symbol: B3GAT1
• Gene ID (NCBI): 27087
• RRID: AB_2878577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.; Authors - Sihui Yu
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	IF	Front Bioeng Biotechnol; Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.; Authors - Jie Chen
human	IHC	BMC Med Genomics; Establishment and validation of an immune infiltration predictive model for ovarian cancer; Authors - Zhenxia Song
human,mouse	IHC	Aging (Albany NY); Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma; Authors - Xiaonan Zhang