B3GAT1 Monoclonal antibody, PBS Only

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  WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 66711-1-Ig (same clone as 66711-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 66711-1-PBS

Clone No.1F4A11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

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Product Information

66711-1-PBS targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13593

  Product name: Recombinant human CD57 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 25-334 aa of BC010466

  Sequence: HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

• Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa, 100 kDa
• GenBank Accession Number: BC010466
• Gene Symbol: B3GAT1
• Gene ID (NCBI): 27087
• RRID: AB_2882062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2W7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.