B3GNT3 Polyclonal antibody

B3GNT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 18098-1-AP

Synonyms

Beta-1,3-N-acetylglucosaminyltransferase 3, Beta-1,3-Gn-T3, Beta-1,3-GalTase 8, Beta-1,3-galactosyltransferase 8, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase

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Validation Data Gallery

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  WB analysis using 18098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18098-1-AP (B3GNT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18098-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18098-1-AP (B3GNT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18098-1-AP (B3GNT3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 18098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18098-1-AP (B3GNT3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 18098-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using B3GNT3 antibody (18098-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, SH-SY5Y cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 6 publications below

Product Information

18098-1-AP targets B3GNT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT3 fusion protein Ag12061
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
• Calculated Molecular Weight: 372 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC066876
• Gene Symbol: B3GNT3
• Gene ID (NCBI): 10331
• RRID: AB_10596928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) is a type II transmembrane protein located in the Golgi apparatus and contributes to the regulation of L-selectin ligand function, lymphocyte trafficking, and T cell homing. B3GNT3 is overexpressed in breast cancer, and also plays a critical role in the aggressiveness of pancreatic cancer (PMID: 33718036, 30466404). Western blot analysis detected B3GNT3 at an apparent molecular mass of 43 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1.; Authors - Chia-Wei Li
human	IHC	Transl Lung Cancer Res; Identifying the prognostic significance of B3GNT3 with PD-L1 expression in lung adenocarcinoma.; Authors - Xuefeng Leng
human	WB,IHC	PLoS One; B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.; Authors - Weijing Zhang
human	WB	BMC Bioinformatics; A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma; Authors - Jin-Xiao Liang
human	WB,IHC	J Clin Pathol; B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.; Authors - Liuwei Gao
human	IHC	Oncol Lett; B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.; Authors - Kaiwen Kong