B3GNT6 Polyclonal antibody

B3GNT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21291-1-AP

Synonyms

B3Gn T6, B3GNT6, Beta 1,3 Gn T6, Beta3Gn T6, BGnT 6, Core 3 synthase, IMAGE:4907098

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Validation Data Gallery

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  WB analysis using 21291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21291-1-AP (B3GNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21291-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21291-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse small intestine tissue, mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 21291-1-AP is B3GNT6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT6 fusion protein Ag15825
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC103910
• Gene Symbol: B3GNT6
• Gene ID (NCBI): 192134
• RRID: AB_2878834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)

Publications

Species	Application	Title
human	WB,IHC	Oncogene; Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity.; Authors - J Ye
	WB	Mol Ther Methods Clin Dev; An etanercept O-glycovariant with enhanced potency.; Authors - Thomas G Biel
human,mouse	WB,IHC	Cancer Med; G-quadruplex-enhanced circular single-stranded DNA (G4-CSSD) adsorption of miRNA to inhibit colon cancer progression; Authors - Haidong Wu
rat	WB	Int J Food Sci Nutr; Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats; Authors - Bo Peng