Tested Applications
| Positive WB detected in | Jurkat cells, COLO 320 cells, mouse placenta tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse colon tissue, rat stomach tissue, human colon cancer tissue, human placenta tissue, rat colon tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22879-1-AP targets B3GNT7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18890 Product name: Recombinant human B3GNT7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 31-164 aa of BC148680 Sequence: PGQFLQEPPPPTLEPQKAQKPNGQLVNPNNFWKNPKDVAAPTPMASQGPQAWDVTTTNCSANINLTHQPWFQVLEPQFRQFLFYRHCRYFPMLLNHPEKCRGDVYLLVVVKSVITQHDRREAIRQTWGRERQSA Predict reactive species |
| Full Name | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 |
| Calculated Molecular Weight | 330 aa, 37 kDa |
| Observed Molecular Weight | 40-46 kDa |
| GenBank Accession Number | BC148680 |
| Gene Symbol | B3GNT7 |
| Gene ID (NCBI) | 93010 |
| RRID | AB_2879182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for B3GNT7 antibody 22879-1-AP | Download protocol |
| WB protocol for B3GNT7 antibody 22879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B3GNT7 Polyclonal antibody (Cat# 22879-1-AP) has a total of 1 citation. It appears in Clinical and Experimental Medicine, where it was used for immunofluorescence (IF) in human and mouse samples. The cited study investigates B3GNT7's role in preserving intestinal barrier integrity as a defense mechanism against acute pancreatitis progression, placing the research in the fields of gastroenterology and intestinal biology.
| Species | Application | Title |
|---|---|---|
Clin Exp Med B3GNT7 preserves intestinal barrier integrity: a key defense mechanism against acute pancreatitis progression. |
