B3GNT7 Polyclonal antibody

B3GNT7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22879-1-AP

Synonyms

BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7



Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, mouse placenta tissue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22879-1-AP targets B3GNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GNT7 fusion protein Ag18890
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC148680
Gene Symbol B3GNT7
Gene ID (NCBI) 93010
RRIDAB_2879182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).

Protocols

Product Specific Protocols
WB protocol for B3GNT7 antibody 22879-1-APDownload protocol
IHC protocol for B3GNT7 antibody 22879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols