B3GNT7 Polyclonal antibody

B3GNT7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22879-1-AP

Synonyms

BGnT7, Beta3Gn-T7, beta3GnT7, Beta-1,3-N-acetylglucosaminyltransferase 7, Beta-1,3-Gn-T7

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Validation Data Gallery

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  WB analysis of Jurkat using 22879-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22879-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 22879-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22879-1-AP (B3GNT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22879-1-AP (B3GNT7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 22879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22879-1-AP (B3GNT7 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, COLO 320 cells, mouse placenta tissue
• Positive IHC detected in: human colon cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22879-1-AP targets B3GNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GNT7 fusion protein Ag18890
• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC148680
• Gene Symbol: B3GNT7
• Gene ID (NCBI): 93010
• RRID: AB_2879182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GNT7, the glycosyltransferase gene that was most significantly upregulated by IL-22 treatment, encodes an N-acetylglucosaminyltransferase involved in the biosynthesis of polyLacNAc repeats of keratan sulfate (PMID: 34864058). B3GNT7 was initially identified in 2002 and is notable for high expression in healthy intestinal epithelial cells (PMID: 38886669). Overexpressed B3GNT7 were correlated with poor prognosis in breast cancer patients based on public datasets. B3GNT7 was shown to be a potential biomarker for unfavorable outcomes and therapeutic target of breast cancer (PMID: 37740763).