Tested Applications
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30691-1-AP targets CD86 in WB, IHC, IF, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33514 Product name: Recombinant mouse Cd86 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-244 aa of NM_019388 Sequence: VSVETQAYFNGTAYLPCPFTKAQNISLSELVVFWQDQQKLVLYEHYLGTEKLDSVNAKYLGRTSFDRNNWTLRLHNVQIKDMGSYDCFIQKKPPTGSIILQQTLTELSVIANFSEPEIKLAQNVTGNSGINLTCTSKQGHPKPKKMYFLITNSTNEYGDNMQISQDNVTELFSISNSLSLSFPDGVWHMTVVCVLETESMKISSKPLNFTQEFPSPQTYWK Predict reactive species |
| Full Name | CD86 antigen |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | NM_019388 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| RRID | AB_3086387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42082-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD86 antibody 30691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD86 Polyclonal antibody (Cat# 30691-1-AP) has accumulated 82 citations published in journals including Advanced Materials, Bioactive Materials, Nature Communications, ACS Nano, Biomaterials, Journal of Controlled Release, Advanced Science, Acta Biomaterialia, Materials Today Bio, and International Immunopharmacology. Key research fields include macrophage M1/M2 polarization, immunomodulation, diabetic wound healing, bone regeneration, neuroinflammation, myocardial infarction repair, rheumatoid arthritis, colitis, cancer immunotherapy, and biomaterials-based tissue engineering.
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