B7-H3/CD276 Polyclonal antibody

B7-H3/CD276 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 30052-1-AP


4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30052-1-AP targets B7-H3/CD276 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name CD276 molecule
Calculated Molecular Weight57 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberNM_001024736
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).


Product Specific Protocols
WB protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
IHC protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
IF protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols