BACH1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82842-7-RR (same clone as 82842-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82842-7-RR (same clone as 82842-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

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  ChIP experiment of K-562 using 82842-7-RR (same clone as 82842-7-PBS)

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of BACH1 (82842-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

BACH1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Cat No. 82842-7-PBS

Clone No.4K14

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Synonyms

4K14, BACH 1, BACH-1

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Product Information

82842-7-PBS targets BACH1 in WB, IF/ICC, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5128

  Product name: Recombinant human BACH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 387-736 aa of BC063307

  Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE

• Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC063307
• Gene Symbol: BACH1
• Gene ID (NCBI): 571
• ENSEMBL Gene ID: ENSG00000156273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14867
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.