BAG1S/1M/1L Polyclonal antibody

• Featured Product
• KD/KO Validated

BAG1S/1M/1L Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16150-1-AP

Synonyms

BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic

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Validation Data Gallery

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  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16150-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16150-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16150-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16150-1-AP

  IP result of anti-BAG1S/1M/1L (IP:16150-1-AP, 4ug; Detection:16150-1-AP 1:800) with Jurkat cells lysate 1320 ug.

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  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16150-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells, mouse liver tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

16150-1-AP targets BAG1S/1M/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: BCL2-associated athanogene
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 46-50 kDa, 41-46 kDa, 25-33 kDa
• GenBank Accession Number: NM_004323
• Gene Symbol: BAG1
• Gene ID (NCBI): 573
• RRID: AB_2062288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

Publications

Species	Application	Title
human	WB,IHC	Tumour Biol; Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.; Authors - Wenbai Huang ; KD Validated