BAG2 Polyclonal antibody

• 

  WB analysis using 29820-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29820-1-AP (BAG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

BAG2 Polyclonal Antibody for WB, ELISA

Cat No. 29820-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIAA0576, BAG-2

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, U-251 cells, U-87 MG cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29820-1-AP targets BAG2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAG2 fusion protein Ag30873
• Full Name: BCL2-associated athanogene 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: NM_004282
• Gene Symbol: BAG2
• Gene ID (NCBI): 9532
• RRID: AB_2918348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95816
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).

Publications

Species	Application	Title
human	WB	iScience; A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contacts; Authors - Maoge Zhou