Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13913-1-AP targets SODD/BAG4 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, artemia sinica |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4905 Product name: Recombinant human BAG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-457 aa of BC038505 Sequence: ARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKELLELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL Predict reactive species |
| Full Name | BCL2-associated athanogene 4 |
| Calculated Molecular Weight | 457 aa, 50 kDa |
| Observed Molecular Weight | 60 kDa, 66-70 kDa |
| GenBank Accession Number | BC038505 |
| Gene Symbol | SODD/BAG4 |
| Gene ID (NCBI) | 9530 |
| RRID | AB_2243308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| IP protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| WB protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SODD/BAG4 Polyclonal antibody (Cat# 13913-1-AP) has 3 total citations published in Nature Communications, Molecular Medicine, and Gene. The associated research spans epigenetic regulation of brain stress response in Drosophila, mitophagy and tumorigenesis in colorectal cancer, and functional characterization of As-kip2 during diapause embryo restarting in Artemia sinica. Applications cited include WB, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
Nat Commun Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila | ||
Mol Med USP14 inhibits mitophagy and promotes tumorigenesis and chemosensitivity through deubiquitinating BAG4 in microsatellite instability-high colorectal cancer | ||
Gene Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica. |
