IRSp53 Polyclonal antibody

IRSp53 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11087-2-AP

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSp53, IRSp53/58, Protein BAP2



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11087-2-AP targets IRSp53 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRSp53 fusion protein Ag1552
Full Name BAI1-associated protein 2
Calculated molecular weight 61 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC014020
Gene symbol BAIAP2
Gene ID (NCBI) 10458
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).

Protocols

Product Specific Protocols
WB protocol for IRSp53 antibody 11087-2-APDownload protocol
IHC protocol for IRSp53 antibody 11087-2-APDownload protocol
IP protocol for IRSp53 antibody 11087-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

JCI Insight

Disruption of Robo2-Baiap2 integrated signaling drives cystic disease.

Authors - Qinggang Li
hamsterWB,IP

Proteomics

Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell-Cell Transmission.

Authors - Fei-Long Yu
mouseWB

Hum Mol Genet

Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

Authors - Sean-Patrick Riechers

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

anwesh (Verified Customer) (10-28-2019)

Bands detected at the correct molecular weight. Great antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T, U2OS, A549
IRSp53 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, A549 (Cat no:11087-2-AP)