BAIAP2L2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BAIAP2L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16738-1-AP

Synonyms

UNQ9336/PRO34007, Planar intestinal- and kidney-specific BAR domain protein, Pinkbar, Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2, BAR/IMD domain-containing adapter protein 2-like 2

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Validation Data Gallery

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  WB analysis using 16738-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 16738-1-AP (BAIAP2L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16738-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16738-1-AP (BAIAP2L2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 16738-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16738-1-AP (BAIAP2L2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

16738-1-AP targets BAIAP2L2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAIAP2L2 fusion protein Ag9904
• Full Name: BAI1-associated protein 2-like 2
• Calculated Molecular Weight: 518 aa, 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC015619
• Gene Symbol: BAIAP2L2
• Gene ID (NCBI): 80115
• RRID: AB_2878309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; BAIAP2L2 is required for the maintenance of mechanotransducing stereocilia of cochlear hair cells.; Authors - Keji Yan ; KO Validated
human	WB	Biomed Pharmacother; BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways.; Authors - Jianing Liu ; KD Validated