BAP1 Polyclonal antibody

BAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10398-1-AP

Synonyms

BAP1, BRCA1 associated protein 1, Cerebral protein 6, HUCEP 13, hucep 6, KIAA0272, UCHL2

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Validation Data Gallery

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  WB analysis of A2780 using 10398-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10398-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10398-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10398-1-AP

  IP result of anti-BAP1 (IP:10398-1-AP, 4ug; Detection:10398-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A2780 cells, human placenta tissue, mouse ovary tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

10398-1-AP targets BAP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAP1 fusion protein Ag0597
• Full Name: BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• Calculated Molecular Weight: 81-91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC001596
• Gene Symbol: BAP1
• Gene ID (NCBI): 8314
• RRID: AB_2180460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA1-associated protein-1 (BAP1),also named as KIAA0272, hucep-6, DKFZp686N04275, FLJ35406, FLJ37180, HUCEP-13, is a deubiquitinating enzyme whose function in the control of the cell cycle requires both its deubiquitinating activity and nuclear localization(PMID:22374320). It helps to control cell proliferation by regulating HCFC1 protein levels and by associating with genes involved in the G(1)-S transition)(PMID:19188440). Defects in BAP1 may be a cause of mesothelioma malignant (MESOM)(PMID:21642991) and tumor predisposition syndrome (TPDS)(PMID:21874003). The all BAP1s except those of C. quinquefasciatus have a molecular mass of more than 90 kDa(PMID:22374320).

Publications

Species	Application	Title
human,mouse	WB,IHC	J Exp Clin Cancer Res; BAP1 regulates HSF1 activity and cancer immunity in pancreatic cancer; Authors - Weiwei Yuan
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB	iScience; Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4; Authors - Chenggang Zhang
mouse	WB	Nature; Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase; Authors - Weiran Ge