BARHL2 Polyclonal antibody

BARHL2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23976-1-AP

Synonyms

BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2



Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23976-1-AP is BARHL2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BARHL2 fusion protein Ag21156
Full Name BarH-like homeobox 2
Calculated molecular weight 387 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC126439
Gene symbol BARHL2
Gene ID (NCBI) 343472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

Protocols

Product Specific Protocols
WB protocol for BARHL2 antibody 23976-1-APDownload protocol
IHC protocol for BARHL2 antibody 23976-1-APDownload protocol
IF protocol for BARHL2 antibody 23976-1-APDownload protocol
Standard Protocols
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