BARHL2 Polyclonal antibody

BARHL2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23976-1-AP

Synonyms

BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2

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Validation Data Gallery

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  WB analysis of HepG2 using 23976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23976-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23976-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23976-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 23976-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23976-1-AP (BARHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23976-1-AP is BARHL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BARHL2 fusion protein Ag21156
• Full Name: BarH-like homeobox 2
• Calculated Molecular Weight: 387 aa, 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC126439
• Gene Symbol: BARHL2
• Gene ID (NCBI): 343472
• RRID: AB_2879386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.