DDX39B Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX39B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14798-1-AP

Synonyms

ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56

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Validation Data Gallery

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14798-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14798-1-AP

  IP result of anti-DDX39B (IP:14798-1-AP, 4ug; Detection:14798-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat spleen tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14798-1-AP targets DDX39B in WB, IHC, IF, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX39B fusion protein Ag6512
• Full Name: HLA-B associated transcript 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000361
• Gene Symbol: BAT1
• Gene ID (NCBI): 7919
• RRID: AB_2061854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	IF,ChIP	Genes Dev; UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide.; Authors - Carmen Pérez-Calero
human,mouse	WB,IHC	Cell Death Discov; DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.; Authors - Haonan Zhang ; KD Validated
human	WB	iScience; THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate; Authors - Mareike Polenkowski
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang
human	WB,IF,IP	Sci Rep; ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.; Authors - Ashley Boehringer