BBOF1 Polyclonal antibody, PBS Only

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• BBOF1 Polyclonal antibody, PBS Only (24337-1-PBS)

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  WB analysis of TT using 24337-1-AP (same clone as 24337-1-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 24337-1-AP (CCDC176 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

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  WB analysis using 24337-1-AP (same clone as 24337-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

BBOF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24337-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

24337-1-PBS targets BBOF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19302

  Product name: Recombinant human C14orf45 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 12-229 aa of BC104981

  Sequence: LQQHAMIENQAGQVEIDKLQHLLQMKDREMNRVKKLAKNILDERTEVERFFLDALHQVKQQILISRKHYKQIAQAAFNLKMRAACTGRTEYPKIRTFDGREHSTNSVNQDLLEAEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSRPPVPDYVVSDSGETKEFGDESKLQDKIFITQQIAISDSSGEVVLPTIPKEPQESDTGTF

• Full Name: chromosome 14 open reading frame 45
• Calculated Molecular Weight: 529 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC104981
• Gene Symbol: BBOF1
• Gene ID (NCBI): 80127
• RRID: AB_3669433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8ND07
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.