BBS10 Monoclonal antibody, PBS Only

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  WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

BBS10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66679-1-PBS

Clone No.4C6A10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

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Product Information

66679-1-PBS targets BBS10 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17960

  Product name: Recombinant human BBS10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 573-723 aa of BC013795

  Sequence: MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL

• Full Name: Bardet-Biedl syndrome 10
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013795
• Gene Symbol: BBS10
• Gene ID (NCBI): 79738
• RRID: AB_2882033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TAM1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).