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- KD/KO Validated
BBS4 Polyclonal antibody
BBS4 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 12766-1-AP
|Positive WB detected in||HEK-293 cells, mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 8 publications below|
|IF||See 9 publications below|
12766-1-AP targets BBS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS4 fusion protein Ag3430|
|Full Name||Bardet-Biedl syndrome 4|
|Calculated molecular weight||519 aa, 58 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC027624|
|Gene ID (NCBI)||585|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for BBS4 antibody 12766-1-AP||Download protocol|
|IHC protocol for BBS4 antibody 12766-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.
The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.
Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.
Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.
J Biol Chem
DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christine (Verified Customer) (01-27-2023)
Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker