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  • KD/KO Validated

BBS4 Polyclonal antibody

BBS4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12766-1-AP

Synonyms

Bardet Biedl syndrome 4, BBS4



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12766-1-AP targets BBS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS4 fusion protein Ag3430
Full Name Bardet-Biedl syndrome 4
Calculated molecular weight 519 aa, 58 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC027624
Gene symbol BBS4
Gene ID (NCBI) 585
RRIDAB_10596774
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

Protocols

Product Specific Protocols
WB protocol for BBS4 antibody 12766-1-APDownload protocol
IHC protocol for BBS4 antibody 12766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Authors - Dragomir B Krastev
mouseWB,IF

PLoS Biol

Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.

Authors - Naila Haq
  • KO Validated
humanIF

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
mouseIF

Autophagy

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Authors - Andreas Struchtrup
humanWB

EMBO Rep

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Authors - Melis D Arslanhan
mouseWB

Elife

Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.

Authors - Yan Xiong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (01-27-2023)

Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HEK293T