|Positive WB detected in||HEK-293 cells, mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12766-1-AP targets BBS4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS4 fusion protein Ag3430|
|Full Name||Bardet-Biedl syndrome 4|
|Calculated molecular weight||519 aa, 58 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC027624|
|Gene ID (NCBI)||585|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.
BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors.
Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.
J Biol Chem
DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.
BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1.