• Featured Product
  • KD/KO Validated

BBS4 Polyclonal antibody

BBS4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12766-1-AP

Synonyms

Bardet Biedl syndrome 4, BBS4



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12766-1-AP targets BBS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS4 fusion protein Ag3430
Full Name Bardet-Biedl syndrome 4
Calculated molecular weight 519 aa, 58 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC027624
Gene symbol BBS4
Gene ID (NCBI) 585
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BBS4 antibody 12766-1-APDownload protocol
IHC protocol for BBS4 antibody 12766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Authors - Ezgi Odabasi
mouseWB,IF

PLoS Biol

Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.

Authors - Naila Haq
  • KO Validated
ratWB

Sci Rep

BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors.

Authors - Qingwen Xu
  • KD Validated
humanWB

Sci Rep

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Authors - Deniz Conkar
humanWB,IF

J Biol Chem

DAZ interacting protein 1 (Dzip1) phosphorylation by Polo-like kinase 1 (Plk1) regulates the centriolar satellites localization of the BBSome during the cell cycle.

Authors - Boyan Zhang
mouseWB,IF

Sci Rep

BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1.

Authors - Victoria Prieto-Echagüe
  • KD Validated