BBS5 Monoclonal antibody

BBS5 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4G8E12

Cat no : 66136-1-Ig

Synonyms

Bardet Biedl syndrome 5, BBS5



Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66136-1-Ig is BBS5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BBS5 fusion protein Ag6844
Full Name Bardet-Biedl syndrome 5
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC044593
Gene symbol BBS5
Gene ID (NCBI) 129880
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Protocols

Product Specific Protocols
WB protocol for BBS5 antibody 66136-1-IgDownload protocol
IHC protocol for BBS5 antibody 66136-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols