BBS9 Polyclonal antibody

BBS9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14460-1-AP

Synonyms

B1, Bardet Biedl syndrome 9, BBS9, C18, D1, Protein PTHB1, PTHB1



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, human heart tissue, HeLa cells, Jurkat cells, mouse heart tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14460-1-AP targets BBS9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS9 fusion protein Ag5665
Full Name Bardet-Biedl syndrome 9
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032715
Gene Symbol BBS9
Gene ID (NCBI) 27241
RRIDAB_10598001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BBS9 antibody 14460-1-APDownload protocol
IHC protocol for BBS9 antibody 14460-1-APDownload protocol
IP protocol for BBS9 antibody 14460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
humanWB

EMBO Rep

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Authors - Benjamin Vitre
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha