BBS9 Polyclonal antibody

BBS9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14460-1-AP

Synonyms

B1, Bardet Biedl syndrome 9, BBS9, C18, D1, Protein PTHB1, PTHB1



Tested Applications

Positive WB detected inmouse testis tissue, human heart tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14460-1-AP targets BBS9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS9 fusion protein Ag5665
Full Name Bardet-Biedl syndrome 9
Calculated molecular weight 99 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032715
Gene symbol BBS9
Gene ID (NCBI) 27241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BBS9 antibody 14460-1-APDownload protocol
IHC protocol for BBS9 antibody 14460-1-APDownload protocol
IP protocol for BBS9 antibody 14460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha
humanWB

EMBO Rep

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Authors - Benjamin Vitre
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether