BBS9 Polyclonal antibody

BBS9 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14460-1-AP

Synonyms

B1, Bardet Biedl syndrome 9, BBS9, C18, D1, Protein PTHB1, PTHB1

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Validation Data Gallery

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  WB analysis of mouse testis using 14460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14460-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14460-1-AP

  IP Result of anti-BBS9 (IP:14460-1-AP, 4ug; Detection:14460-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver using 14460-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 14460-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 14460-1-AP

  IF result (cytoplasm stain) of anti-BBS9 (14460-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: mouse testis tissue, human heart tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

14460-1-AP targets BBS9 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS9 fusion protein Ag5665
• Full Name: Bardet-Biedl syndrome 9
• Calculated molecular weight: 99 kDa
• Observed molecular weight: 60 kDa
• GenBank accession number: BC032715
• Gene symbol: BBS9
• Gene ID (NCBI): 27241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
human	WB	EMBO Rep; IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.; Authors - Benjamin Vitre
mouse	WB	Invest Ophthalmol Vis Sci; Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.; Authors - Satyabrata Sinha