BBS9 Polyclonal antibody

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  WB analysis using 14460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14460-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14460-1-AP

  IP result of anti-BBS9 (IP:14460-1-AP, 4ug; Detection:14460-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver using 14460-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 14460-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 14460-1-AP

  IF result (cytoplasm stain) of anti-BBS9 (14460-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

BBS9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14460-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Protein PTHB1, Parathyroid hormone-responsive B1 gene protein, BBS 9, Bardet-Biedl syndrome 9 protein, Bardet Biedl syndrome 9

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, human heart tissue, HeLa cells, Jurkat cells, mouse heart tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

14460-1-AP targets BBS9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS9 fusion protein Ag5665
• Full Name: Bardet-Biedl syndrome 9
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032715
• Gene Symbol: BBS9
• Gene ID (NCBI): 27241
• RRID: AB_10598001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3SYG4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
human	WB	EMBO Rep; IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.; Authors - Benjamin Vitre
mouse	WB	Invest Ophthalmol Vis Sci; Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.; Authors - Satyabrata Sinha
		PLoS Biol; Melanocortin 1 receptor mediates melanin production by interacting with the BBSome in primary cilia; Authors - Xiaoyu Tian