Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue, human heart tissue, HeLa cells, Jurkat cells, mouse heart tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14460-1-AP targets BBS9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5665 Product name: Recombinant human BBS9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-310 aa of BC032715 Sequence: MGYLRIFSPHPAKTGDGAQAEDLLLEVDLRDPVLQVEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQCQMKLMYEHNLQRTACNMTYGSFGGVKGRDLICIQSMDGMLMVFEQESYAFGRFLPGFLLPGPLAYSSRTDSFLTVSSCQQVESYKYQVLAFATDADKRQETEQQKLGSGKRLVVDWTLNIGEQALDICIVSFNQSASSVFVLGERNFFCLKDNGQIRFMKKLDWSPSCFLPYCSVSEGTINTLIGNHNNMLHIYQDVTLKWATQLPHIPVAVRVGCLQFSLWKHLLPRSSTLEK Predict reactive species |
| Full Name | Bardet-Biedl syndrome 9 |
| Calculated Molecular Weight | 99 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC032715 |
| Gene Symbol | BBS9 |
| Gene ID (NCBI) | 27241 |
| RRID | AB_10598001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SYG4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BBS9 antibody 14460-1-AP | Download protocol |
| IP protocol for BBS9 antibody 14460-1-AP | Download protocol |
| WB protocol for BBS9 antibody 14460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Dev Cell IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment. | ||
EMBO Rep IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis. | ||
Mol Neurobiol SnRNA-seq Interprets Mechanisms by which Three Glial Cell Types Influence Myelin Regeneration in Adult Drug-Resistant Epilepsy-Related Cognitive Impairment | ||
Invest Ophthalmol Vis Sci Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis. | ||
PLoS Biol Melanocortin 1 receptor mediates melanin production by interacting with the BBSome in primary cilia | ||
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lola (Verified Customer) (10-23-2025) | This antibody works well in immunofluorescence and expansion microscopy. Perfect for cilia and BBSome imaging. We use cold MeOH fixation before staining.
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