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  • KD/KO Validated

BBX Polyclonal antibody

BBX Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17254-1-AP

Synonyms

BBX, Bobby sox homolog, bobby sox homolog (Drosophila), HBP2, HMG box containing protein 2, HSPC339, MDS001



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17254-1-AP targets BBX in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBX fusion protein Ag10691
Full Name bobby sox homolog (Drosophila)
Calculated molecular weight 941 aa, 105 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC012837
Gene symbol BBX
Gene ID (NCBI) 56987
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature

Protocols

Product Specific Protocols
WB protocol for BBX antibody 17254-1-APDownload protocol
IHC protocol for BBX antibody 17254-1-APDownload protocol
IP protocol for BBX antibody 17254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIHC

Dev Genes Evol

Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic.

Authors - TieLin Chen
ratWB,IHC,IF

Biol Reprod

PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.

Authors - Chieh-Ying Wang
  • KO Validated