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  • KD/KO Validated

BBX Polyclonal antibody

BBX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 17254-1-AP


BBX, Bobby sox homolog, bobby sox homolog (Drosophila), HBP2, HMG box containing protein 2, HSPC339, MDS001

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17254-1-AP targets BBX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBX fusion protein Ag10691
Full Name bobby sox homolog (Drosophila)
Calculated Molecular Weight 941 aa, 105 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC012837
Gene Symbol BBX
Gene ID (NCBI) 56987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature


Product Specific Protocols
WB protocol for BBX antibody 17254-1-APDownload protocol
IHC protocol for BBX antibody 17254-1-APDownload protocol
IP protocol for BBX antibody 17254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biol Reprod

PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.

Authors - Chieh-Ying Wang
  • KO Validated

Dev Genes Evol

Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic.

Authors - TieLin Chen

Dev Comp Immunol

Discovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure

Authors - Danixa Martínez