Tested Applications
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17254-1-AP targets BBX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10691 Product name: Recombinant human BBX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-597 aa of BC012837 Sequence: KPEDSDCHRKIETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRSSGKGNSSDHEGCWNEESWTFSQSGTSGSKKFKKTKPKEDCLLGSAKLDEEFEKKFNSLPQYSPVTFDRKCVPVPRKKKKTGNVSSEPTKTSKGPFQSQKKNLFHKIVSKYKHKKEKPNVPEKGSGDKWSNKQLFLDAIHPTEAIFSEDRNTMEPVHKVKNIPSIFNTPEPTTTQEPLVGSQKRKARKTKITHLVRTADGRVSPAGGTLDDKPKEQLQRSLPKATETDCNDKCSHNTEVGETRSSTPEMPAVSAFFSLAALAEVAAMENVHRGQRSTPLTHDGQPKEMPQAPVLISCADQ Predict reactive species |
| Full Name | bobby sox homolog (Drosophila) |
| Calculated Molecular Weight | 941 aa, 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC012837 |
| Gene Symbol | BBX |
| Gene ID (NCBI) | 56987 |
| RRID | AB_2065285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WY36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BBX antibody 17254-1-AP | Download protocol |
| IP protocol for BBX antibody 17254-1-AP | Download protocol |
| WB protocol for BBX antibody 17254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BBX polyclonal antibody (Cat# 17254-1-AP) has 4 citations published in Experimental and Molecular Medicine, Biology of Reproduction, Developmental and Comparative Immunology, and Developmental Genes and Evolution. The research fields span phosphate homeostasis and FGF23 regulation, growth and male fertility, bacterial and thermal stress responses in fish, and HMG-box transcription factor characterization. Applications cited include WB, IHC, and IF across mouse, rat, and zebrafish species.
| Species | Application | Title |
|---|---|---|
Exp Mol Med The transcription factor BBX regulates phosphate homeostasis through the modulation of FGF23 | ||
Biol Reprod PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.
| ||
Dev Comp Immunol Discovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure | ||
Dev Genes Evol Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic. |
