BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28222-1-AP

Synonyms

AU, Auberger B antigen, Basal cell adhesion molecule, BCAM, CD239, F8/G253 antigen, LU, Lutheran antigen, MSK19

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Validation Data Gallery

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  WB analysis using 28222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28222-1-AP (BCAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HT-29 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28222-1-AP targets BCAM in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCAM fusion protein Ag28181
• Full Name: basal cell adhesion molecule (Lutheran blood group)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67-80 kDa
• GenBank Accession Number: BC050450
• Gene Symbol: BCAM
• Gene ID (NCBI): 4059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. The significance of Lu/BCAM on tumorigenesis has been increasingly recognized. Lu/BCAM is involved in cell-cell adhesion and migration in skin tumor (PMID: 11121140, PMID: 10728810). Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705).