Brevican Polyclonal antibody

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• Brevican Polyclonal antibody (19017-1-AP)

Brevican Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19017-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

BCAN, UNQ2525/PRO6018, CSPG7, Brevican core protein, Brain-enriched hyaluronan-binding protein

• 

  WB analysis of human brain using 19017-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 19017-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 19017-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Y79 using 19017-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cerebellum using 19017-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cerebellum using 19017-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 19017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, COLO 320 cells, SH-SY5Y cells, Y79 cells
• Positive IHC detected in: human cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19017-1-AP targets Brevican in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13552

  Product name: Recombinant human BCAN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-350 aa of BC010571

  Sequence: LEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPTYPASLTDVSLALNELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVY

• Full Name: brevican
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 145 kDa, 72kDa, 80 kDa
• GenBank Accession Number: BC010571
• Gene Symbol: BCAN
• Gene ID (NCBI): 63827
• RRID: AB_10643526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96GW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

Publications

What published studies show

The Brevican Polyclonal antibody (Cat# 19017-1-AP) has 6 total citations published in Theranostics, Molecular Psychiatry, British Journal of Pharmacology, PLoS Biology, FASEB Journal, and Research Square. The research fields span neurorepair after spinal cord injury, hippocampal molecular profiling in autism spectrum disorder, perineuronal net remodeling under chronic stress, retrosplenial cortical reorganization and memory circuit instability during adolescence, and the role of meprins in brain extracellular matrix and perineuronal nets.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IF	Theranostics; Biomimetic core-shell GelMA microspheres co-delivering ANXA1, NGF, and fibronectin enable phase-matched immunomodulation and neurorepair after spinal cord injury.; Authors - Youjun Liu
Human		Mol Psychiatry; Molecular profiling of the hippocampus of children with autism spectrum disorder; Authors - Lindsay E Rexrode
		Br J Pharmacol; Subregion-specific perineuronal net remodelling in the dorsal hippocampus drives vulnerability and resilience to chronic stress: The role of venlafaxine treatment.; Authors - Paola Brivio
		PLoS Biol; Retrosplenial cortical reorganization during late adolescence introduces instability of contextual memory circuits.; Authors - Hui Zhang
		FASEB J; The Role of Meprins on the Brain Extracellular Matrix and Perineuronal Nets.; Authors - Simon Kreiselmaier
		Res Sq; Retrosplenial Cortical Reorganization During Late Adolescence Introduces Instability of Episodic Memory Circuits; Authors - Jelena Radulovic