BCAR3 Polyclonal antibody

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• BCAR3 Polyclonal antibody (13628-1-AP)

BCAR3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13628-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

AND-34, BCAR 3, Breast cancer anti-estrogen resistance protein 3, KIAA0554, MIG7

• 

  WB analysis using 13628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13628-1-AP (BCAR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat adrenal gland using 13628-1-AP

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13628-1-AP (BCAR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IHC detected in: rat adrenal gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13628-1-AP targets BCAR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4546

  Product name: Recombinant human BCAR3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 475-825 aa of BC039895

  Sequence: YLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL

• Full Name: breast cancer anti-estrogen resistance 3
• Calculated Molecular Weight: 825 aa, 93 kDa
• Observed Molecular Weight: 92-105 kDa
• GenBank Accession Number: BC039895
• Gene Symbol: BCAR3
• Gene ID (NCBI): 8412
• RRID: AB_2877962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The BCAR3 Polyclonal antibody (Cat# 13628-1-AP) has 1 total citation. It appears in International Journal of Nanomedicine (2024), in a study on localized Bcar3 siRNA delivery via nano-self-assembly to treat idiopathic pulmonary fibrosis by disrupting macrophage-fibroblast crosstalk. The associated research fields include nanomedicine, pulmonary fibrosis, and cell-cell crosstalk. The antibody was used for immunofluorescence (IF) in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Int J Nanomedicine; Localized Administration of Bcar3 siRNA via Nano-Self-Assembly to Treat Idiopathic Pulmonary Fibrosis by Disrupting Macrophage-Fibroblast Crosstalk; Authors - Chenxi Zeng