BCAT2 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 68586-1-Ig (same clone as 68586-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68586-1-Ig (same clone as 68586-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50268-2

  Cytometric bead array standard curve of MP50268-2, BCAT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68586-1-PBS. Detection antibody: 68586-4-PBS. Standard:Ag9595. Range: 0.781-100 ng/mL.

BCAT2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68586-1-PBS

Clone No.1A5A5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

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Product Information

68586-1-PBS targets BCAT2 as part of a matched antibody pair:

MP50268-2: 68586-1-PBS capture and 68586-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9595

  Product name: Recombinant human BCAT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 41-392 aa of BC001900

  Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

• Full Name: branched chain aminotransferase 2, mitochondrial
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC001900
• Gene Symbol: BCAT2
• Gene ID (NCBI): 587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15382
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).