BCHE Polyclonal antibody

BCHE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23854-1-AP

Synonyms

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23854-1-AP is BCHE Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCHE fusion protein Ag20888
Full Name butyrylcholinesterase
Calculated molecular weight 602 aa, 68 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC008396
Gene symbol BCHE
Gene ID (NCBI) 590
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

Protocols

Product Specific Protocols
WB protocol for BCHE antibody 23854-1-APDownload protocol
IHC protocol for BCHE antibody 23854-1-APDownload protocol
IF protocol for BCHE antibody 23854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Biomed Eng

Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose.

Authors - Yuanyuan Li