BCKDHB Polyclonal antibody

BCKDHB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, RIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13685-1-AP

Synonyms

BCKDHB, E1B

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Validation Data Gallery

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  WB analysis using 13685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13685-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB Antibody) at dilution of 1:350 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, Transfected HEK-293 cells, rat liver tissue
• Positive IHC detected in: human colon cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 4 publications below
• RIP: See 1 publications below

Product Information

13685-1-AP targets BCKDHB in WB, IHC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCKDHB fusion protein Ag4552
• Full Name: branched chain keto acid dehydrogenase E1, beta polypeptide
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC040139
• Gene Symbol: BCKDHB
• Gene ID (NCBI): 594
• RRID: AB_2877972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)

Publications

Species	Application	Title
human	IHC	Nat Cancer; Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers; Authors - Baijun Dong
sheep	WB	Anim Sci J; MiR-330-5p negatively regulates ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2.; Authors - Tao Shi
mouse	WB	Cell Rep; SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis.; Authors - Elma Zaganjor
human	WB	Cell Rep; Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression; Authors - Dongdong Yang
human	IHC	Nat Commun; AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis; Authors - Yanan Wang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang