BCKDK Polyclonal antibody

BCKDK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15718-1-AP

Synonyms

BCKDK

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Validation Data Gallery

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  WB analysis of mouse pancreas using 15718-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 15718-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15718-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15718-1-AP targets BCKDK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCKDK fusion protein Ag8395
• Full Name: branched chain ketoacid dehydrogenase kinase
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC007363
• Gene Symbol: BCKDK
• Gene ID (NCBI): 10295
• RRID: AB_2878174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.; Authors - Yang Yang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury; Authors - Xiao Han