BCL10 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 85858-2-RR (same clone as 85858-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85858-2-RR (BCL10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85858-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02160-2

  The mean BCL10 concentration was determined to be 105.67 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 327.67 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load and 82.11 ng/mL in A549 cell extract based on a 1.10 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02160-2

  Sandwich ELISA standard curve of MP02160-2, Human BCL10 Recombinant Matched Antibody Pair - PBS only. 85858-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12170. 85858-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP02160-2

  Cytometric bead array standard curve of MP02160-2, BCL10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85858-3-PBS. Detection antibody: 85858-2-PBS. Standard: Ag12170. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85858-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85858-2-RR against Human BCL10 were performed. The affinity constant is below 1 pM.

BCL10 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 85858-2-PBS

Clone No.250149A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

B-cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, Bcl 10, Bcl-10, c E10

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Product Information

85858-2-PBS targets BCL10 as part of a matched antibody pair:

MP02160-2: 85858-3-PBS capture and 85858-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12170

  Product name: Recombinant human BCL10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-233 aa of BC053617

  Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ

• Full Name: B-cell CLL/lymphoma 10
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC053617
• Gene Symbol: BCL10
• Gene ID (NCBI): 8915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95999
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.