BCL7B Polyclonal antibody

• Featured Product
• KD/KO Validated

BCL7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11740-1-AP

Synonyms

B cell CLL/lymphoma 7B

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Validation Data Gallery

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  WB analysis of HeLa using 11740-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11740-1-AP

  WB result of BCL7B antibody (11740-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL7B transfected HeLa cells.

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  WB analysis of human brain using 11740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11740-1-AP (BCL7B antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11740-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11740-1-AP (BCL7B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

11740-1-AP targets BCL7B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL7B fusion protein Ag2333
• Full Name: B-cell CLL/lymphoma 7B
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC000956
• Gene Symbol: BCL7B
• Gene ID (NCBI): 9275
• RRID: AB_2063596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro.; Authors - Daowen Li
mouse	WB	Aging (Albany NY); S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway.; Authors - Zhenxuan Shao
mouse	WB,IHC	Sci Rep; The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.; Authors - Lena Wischhof ; KO Validated
human	WB,CoIP	Life Sci Alliance; BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression; Authors - Nicholas Dietrich ; KD Validated