Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11740-1-AP targets BCL7B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2333 Product name: Recombinant human BCL7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC000956 Sequence: MSGRSVRAETRSRAKDDIKKVMAAIEKVRKWEKKWVTVGDTSLRIFKWVPVTDSKEKEKSKSNSSAAREPNGFPSDASANSSLLLEFQDENSNQSSVSDVYQLKVDSSTNSSPSPQQSESLSPAHTSDFRTDDSQPPTLGQEILEEPSLPSSEVADEPPTLTKEEPVPLETQVVEEEEDSGAPPLKRFCVDQPTVPQTASES Predict reactive species |
| Full Name | B-cell CLL/lymphoma 7B |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC000956 |
| Gene Symbol | BCL7B |
| Gene ID (NCBI) | 9275 |
| RRID | AB_2063596 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQE9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BCL7B antibody 11740-1-AP | Download protocol |
| IHC protocol for BCL7B antibody 11740-1-AP | Download protocol |
| WB protocol for BCL7B antibody 11740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-BCL7A antibody (Cat# 11740-1-AP) has 6 total citations. These appear in Nucleic Acids Research, Oxidative Medicine and Cellular Longevity, Aging (Albany NY), Scientific Reports, Life Science Alliance, and Experimental Hematology. Research fields include nucleosome structure, oxidative stress and liver damage, osteoarthritis, motor coordination and Purkinje cell function, chromatin remodeling and gene expression, and hematopoiesis. Applications span WB, IHC, and CoIP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro. | ||
Aging (Albany NY) S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway. | ||
Sci Rep The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.
| ||
Life Sci Alliance BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
| ||
Exp Hematol Bcl7b and Bcl7c Subunits of BAF Chromatin Remodeling Complexes are Largely Dispensable for Hematopoiesis |
