BCMO1 Polyclonal antibody

BCMO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23308-1-AP

Synonyms

BCMO1, Beta carotene dioxygenase 1

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Validation Data Gallery

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  WB analysis of COLO 320 using 23308-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23308-1-AP (BCMO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 23308-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 23308-1-AP (BCMO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23308-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23308-1-AP (BCMO1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse colon tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23308-1-AP is BCMO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCMO1 fusion protein Ag19732
• Full Name: beta-carotene 15,15'-monooxygenase 1
• Calculated Molecular Weight: 547 aa, 63 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC126210
• Gene Symbol: BCMO1
• Gene ID (NCBI): 53630
• RRID: AB_2879252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCMO1(Beta,beta-carotene 15,15'-monooxygenase) is also named as BCDO, BCDO1 and belongs to the carotenoid oxygenase family.The human BCMO1 cDNA encodes a 63-kDa protein with homologies to members of a large and diverse family of polyene chain oxidases and carotenoid cleavage enzymes.Although BCMO1 can be detected in several tissues, its expression is most pronounced in intestinal mucosa and liver(PMID:16504037).It catalyzes the central cleavage of β-carotene to all-trans retinal and is the key enzyme in the intestinal metabolism of carotenes to vitamin A.

Publications

Species	Application	Title
human	WB,IF	Exp Eye Res; Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy; Authors - Binxing Li