BCO2 Polyclonal antibody

BCO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14324-1-AP

Synonyms

B DIOX II, BCO2, Beta carotene dioxygenase 2, beta carotene oxygenase 2

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Validation Data Gallery

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  WB analysis using 14324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14324-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14324-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 14324-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 14324-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells, rat liver tissue
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14324-1-AP targets BCO2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCO2 fusion protein Ag5590
• Full Name: beta-carotene oxygenase 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC041656
• Gene Symbol: BCO2
• Gene ID (NCBI): 83875
• RRID: AB_2227901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

Publications

Species	Application	Title
human, mouse	WB,IHC	Proc Natl Acad Sci U S A; Inactivity of human β,β-carotene-9',10'-dioxygenase (BCO2) underlies retinal accumulation of the human macular carotenoid pigment.; Authors - Binxing Li
mouse	WB	J Nutr Biochem; Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.; Authors - Xin Guo
mouse	WB	Mol Nutr Food Res; Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice.; Authors - Yu Huifeng H
mouse	WB	Mol Nutr Food Res; Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase α2 subunit.; Authors - Dingbo Lin
mouse		Molecules; Carotenoid Lutein Selectively Inhibits Breast Cancer Cell Growth and Potentiates the Effect of Chemotherapeutic Agents through ROS-Mediated Mechanisms.; Authors - Xiaoming Gong
human	WB,IF	Exp Eye Res; Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy; Authors - Binxing Li