BCORL1 Polyclonal antibody

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• BCORL1 Polyclonal antibody (24315-1-AP)

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  WB analysis using 24315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 24315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BCORL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CXorf10

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, HuH-7 cells
• Positive IHC detected in: mouse testis tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24315-1-AP targets BCORL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19522

  Product name: Recombinant human BCORL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC156742

  Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA

• Full Name: BCL6 co-repressor-like 1
• Calculated Molecular Weight: 1711 aa, 183 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC156742
• Gene Symbol: BCORL1
• Gene ID (NCBI): 63035
• RRID: AB_3669432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5H9F3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.