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  • KD/KO Validated

BCR Polyclonal antibody

BCR Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, monkey and More (1)





Cat no : 22585-1-AP


ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, PHL

Tested Applications

Positive WB detected inY79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22585-1-AP is BCR Fusion Protein expressed in E. coli.

Tested Reactivity human, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCR fusion protein Ag18281
Full Name breakpoint cluster region
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC066122
Gene Symbol BCR
Gene ID (NCBI) 613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.


Product Specific Protocols
WB protocol for BCR antibody 22585-1-APDownload protocol
IHC protocol for BCR antibody 22585-1-APDownload protocol
IF protocol for BCR antibody 22585-1-APDownload protocol
IP protocol for BCR antibody 22585-1-APDownload protocol
FC protocol for BCR antibody 22585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Neurogastroenterol Motil

Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.

Authors - Yongtao Xiao
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100