BCR Polyclonal antibody

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• BCR Polyclonal antibody (22585-1-AP)

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• KD/KO Validated

BCR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22585-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Renal carcinoma antigen NY-REN-26, EC:2.7.11.1, breakpoint cluster region, BCR1, BCR 1

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  WB analysis of Y79 using 22585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 22585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 22585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 22585-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 22585-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COS-7 using 22585-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 22585-1-AP

  IP result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 22585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCR antibody (22585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 22585-1-AP

  Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Y79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22585-1-AP targets BCR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18281

  Product name: Recombinant human BCR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC066122

  Sequence: MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQS

• Full Name: breakpoint cluster region
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC066122
• Gene Symbol: BCR
• Gene ID (NCBI): 613
• RRID: AB_2879127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P11274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	Neurogastroenterol Motil; Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.; Authors - Yongtao Xiao ; KO Validated
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100