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BCS1L Monoclonal antibody

BCS1L Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G6H1

Cat no : 60212-1-Ig

Synonyms

BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD



Tested Applications

Positive WB detected inColo320 cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue, human brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60212-1-Ig targets BCS1L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCS1L fusion protein Ag18280
Full Name BCS1-like (yeast)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC007500
Gene Symbol BCS1L
Gene ID (NCBI) 617
RRIDAB_11042318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.

Protocols

Product Specific Protocols
WB protocol for BCS1L antibody 60212-1-IgDownload protocol
IHC protocol for BCS1L antibody 60212-1-IgDownload protocol
IF protocol for BCS1L antibody 60212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols