BDH1 Polyclonal antibody

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  WB analysis using 15417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15417-1-AP

  WB result of BDH1 antibody (15417-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH1 transfected MCF-7 cells.

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  WB analysis of rat liver using 15417-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15417-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15417-1-AP

  IP result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of rat brain using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 15417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using BDH1 antibody (15417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

BDH1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 15417-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30, SDR9C1

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue, mouse heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: rat brain tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

15417-1-AP targets BDH1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7705

  Product name: Recombinant human BDH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-343 aa of BC005844

  Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR

• Full Name: 3-hydroxybutyrate dehydrogenase, type 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC005844
• Gene Symbol: BDH1
• Gene ID (NCBI): 622
• RRID: AB_2274683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02338
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
human	WB	Proc Natl Acad Sci U S A; PMID: 29311302; Authors - Marcus D Goncalves
mouse,human	WB	EMBO Mol Med; Ketone body oxidation increases cardiac endothelial cell proliferation.; Authors - Eva-Maria Weis
mouse	WB	J Cell Biol; CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.; Authors - Désirée Schatton