BDH2 Monoclonal antibody, PBS Only

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  WB analysis using 68279-1-Ig (same clone as 68279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68279-1-Ig (same clone as 68279-1-PBS)

  WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells. This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68279-1-Ig (same clone as 68279-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68279-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68279-1-PBS

Clone No.1B6H5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

(R)-beta-hydroxybutyrate dehydrogenase, 1B6H5, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6

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Product Information

68279-1-PBS targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26108

  Product name: Recombinant human BDH2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-245 aa of BC095414

  Sequence: MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFANEVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL

• Full Name: 3-hydroxybutyrate dehydrogenase, type 2
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC095414
• Gene Symbol: BDH2
• Gene ID (NCBI): 56898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BUT1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.