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Beclin 1 Polyclonal antibody
Beclin 1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 11306-1-AP
Validation Data Gallery
|Positive WB detected in||C6 cells, L02 cells, HEK-293 cells, A431 cells, PC-3 cells, HT-1080 cells, human placenta tissue, HeLa cells, NIH/3T3 cells, Raji cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||MDA-MB-453s cells|
|Positive IHC detected in||human breast cancer tissue, human breast hyperplasia tissue, human prostate hyperplasia tissue, human cervical cancer tissue, human colon cancer tissue, human stomach tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11306-1-AP targets Beclin 1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, chicken, duck, fish, hamster, human, mouse, pig, prawn, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beclin 1 fusion protein Ag1843|
|Full Name||beclin 1, autophagy related|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC010276|
|Gene ID (NCBI)||8678|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.
|Product Specific Protocols|
|WB protocol for Beclin 1 antibody 11306-1-AP||Download protocol|
|IHC protocol for Beclin 1 antibody 11306-1-AP||Download protocol|
|IF protocol for Beclin 1 antibody 11306-1-AP||Download protocol|
|IP protocol for Beclin 1 antibody 11306-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (03-30-2020)
Used for immunoblot, single band at expected molecular weight, good signal to noise ratio
Kanika (Verified Customer) (09-18-2019)
Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments.
Kishor (Verified Customer) (01-30-2019)
Working well for Western Blotting (1:5000) and IF (1:300)