BEND3 Recombinant monoclonal antibody

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• BEND3 Recombinant monoclonal antibody (87001-4-RR)

• 

  WB analysis using 87001-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87001-4-RR (BEND3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87001-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87001-4-RR (BEND3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87001-4-RR

  Biolayer interferometry (BLl) kinetic assays of 87001-4-RR against Human BEND3 were performed. The affinity constant is 6.74 nM.

BEND3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87001-4-RR

Clone No.252025C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, HEK-293 cells, HepG2 cells, U2OS cells, mouse embryo

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87001-4-RR targets BEND3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19137

  Product name: Recombinant human BEND3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 493-828 aa of BC131628

  Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

• Full Name: BEN domain containing 3
• Calculated Molecular Weight: 828 aa, 94 kDa
• Observed Molecular Weight: 94-110 kDa
• GenBank Accession Number: BC131628
• Gene Symbol: BEND3
• Gene ID (NCBI): 57673
• RRID: AB_3745266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T5X7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)