BEND4 Polyclonal antibody

BEND4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24711-1-AP

Synonyms

BEN domain containing 4, BEND4, CCDC4

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Validation Data Gallery

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  WB analysis of mouse testis using 24711-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24711-1-AP (BEND4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24711-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 24711-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 24711-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 24711-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24711-1-AP is BEND4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BEND4 fusion protein Ag20169
• Full Name: BEN domain containing 4
• Calculated Molecular Weight: 534 aa, 58 kDa
• Observed Molecular Weight: 55-58 kDa, 33-36 kDa
• GenBank Accession Number: BC128592
• Gene Symbol: BEND4
• Gene ID (NCBI): 389206
• RRID: AB_2879684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BEND4, also named as CCDC4, has 5 isoforms with MW 55-58 kDa, 45-48 kDa and 36 kDa.