Tested Applications
| Positive WB detected in | mouse liver tissue, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20931-1-AP targets BEND5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14930 Product name: Recombinant human BEND5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 71-421 aa of BC007932 Sequence: ILALAEDKSDLENSVMQKKIKIPKLSLNHVEEDGEVKDYGEEDLQLRHIKRPEGRKPSEVAHKSIEAVVARLEKQNGLSLGHSTCPEEVFVEASPGTEDMDSLEDAVVPRALYEELLRNYQQQQEEMRHLQQELERTRRQLVQQAKKLKEYGALVSEMKELRDLNRRLQDVLLLRLGSGPAIDLEKVKSECLEPEPELRSTFSEEANTSSYYPAPAPVMDKYILDNGKVHLGSGIWVDEEKWHQLQVTQGDSKYTKNLAVMIWGTDVLKNRSVTGVATKKKKDAVPKPPLSPHKLSIVRECLYDRIAQETVDETEIAQRLSKVNKYICEKIMDINKSCKNEERREAKYNLQ Predict reactive species |
| Full Name | BEN domain containing 5 |
| Calculated Molecular Weight | 421 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC007932 |
| Gene Symbol | BEND5 |
| Gene ID (NCBI) | 79656 |
| RRID | AB_2878768 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L4P6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BEND5 antibody 20931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BEND5 Polyclonal antibody (Cat# 20931-1-AP) has 2 citations. One appears in International Journal of Biological Sciences (2022), studying BEND5's role in suppressing breast cancer growth and metastasis via Notch signaling inhibition. The other is in Journal of Thoracic Disease (2023), investigating BEND5 as a novel tumor marker in lung adenocarcinoma. Both studies involve human samples and utilize WB and/or IP applications, covering research fields of cancer biology, tumor signaling, and biomarker discovery.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.
| ||
J Thorac Dis Investigation of the function of the novel tumor marker BEND5 in lung adenocarcinoma based on data mining and in vitro analysis |





