BFSP2 Polyclonal antibody

BFSP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25438-1-AP

Synonyms

49 kDa cytoskeletal protein, BFSP2, CP47, CP49, LIFL L, Phakinin, PHAKOSIN

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Validation Data Gallery

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  WB analysis of mouse eye using 25438-1-AP

  mouse and rat eye tissues were subjected to SDS PAGE followed by western blot with 25438-1-AP (BFSP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse eye and rat eye tissues

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25438-1-AP is BFSP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BFSP2 fusion protein Ag22073
• Full Name: beaded filament structural protein 2, phakinin
• Calculated Molecular Weight: 415 aa, 46 kDa
• Observed Molecular Weight: 45-49 kDa
• GenBank Accession Number: BC113518
• Gene Symbol: BFSP2
• Gene ID (NCBI): 8419
• RRID: AB_2880080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.; Authors - Jeremy A Whitson