MIST1 Polyclonal antibody

MIST1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25140-1-AP

Synonyms

BHLHA15, BHLHB8, MIST 1, MIST1

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Validation Data Gallery

• 

  WB analysis of mouse liver using 25140-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 25140-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 25140-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 25140-1-AP

  IP result of anti-MIST1 (IP:25140-1-AP, 3ug; Detection:25140-1-AP 1:300) with mouse liver tissue lysate 6800ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat pancreas tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25140-1-AP is MIST1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIST1 fusion protein Ag18199
• Full Name: basic helix-loop-helix family, member a15
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC113394
• Gene Symbol: BHLHA15
• Gene ID (NCBI): 168620
• RRID: AB_2879920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.