MIST1 Monoclonal antibody

MIST1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat , Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E10B3

Cat no : 68092-1-Ig

Synonyms

BHLHA15, MIST 1, MIST1

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Validation Data Gallery

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  WB analysis using 68092-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68092-1-Ig (MIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, SW 1990 cells，Pig pancreas tissue, Rat pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68092-1-Ig targets MIST1 in WB, ELISA applications and shows reactivity with Human, Pig, Rat , Mouse samples.

• Tested Reactivity: Human, Pig, Rat , Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MIST1 fusion protein Ag18509
• Full Name: basic helix-loop-helix family, member a15
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC113394
• Gene Symbol: BHLHA15
• Gene ID (NCBI): 168620
• RRID: AB_2918829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Muscle intestine stomach expression 1 (MIST1), also named bHLHa15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (BxPC-3, PANC-1, SW 1990) (PMID:29859299). MIST1 is downregulated in the development of gastric cancer and in gastric cancer cells compared with SW620 cells (PMID:34149921).