BHMT2 Polyclonal antibody

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• BHMT2 Polyclonal antibody (16351-1-AP)

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  WB analysis of mouse liver using 16351-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16351-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16351-1-AP (BHMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16351-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16351-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16351-1-AP (BHMT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BHMT2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16351-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

betaine homocysteine methyltransferase 2, Betaine--homocysteine S-methyltransferase 2, EC:2.1.1.10, S-methylmethionine--homocysteine S-methyltransferase BHMT2, SMM-hcy methyltransferase

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16351-1-AP targets BHMT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9558

  Product name: Recombinant human BHMT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-363 aa of BC020665

  Sequence: MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFAWKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF

• Full Name: betaine-homocysteine methyltransferase 2
• Calculated Molecular Weight: 363 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC020665
• Gene Symbol: BHMT2
• Gene ID (NCBI): 23743
• RRID: AB_2878248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2M3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BHMT2 (Betaine-Homocysteine S-Methyltransferase 2) is a zinc-dependent metalloenzyme that plays a crucial role in regulating amino acid homeostasis and cellular methylation pathways. BHMT2 specifically utilizes S-methylmethionine (SMM)-often called Vitamin U, derived from plant-based foods-as its methyl donor. BHMT2 is most heavily expressed in tissues with strong metabolic activity, particularly the liver and kidneys.