BICD2 Polyclonal antibody

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  WB analysis using 29603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29603-1-AP (BICD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

BICD2 Polyclonal Antibody for WB, ELISA

Cat No. 29603-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

bA526D8.1, Bic D 2, BICD2, KIAA0699, Protein bicaudal D homolog 2

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Tested Applications

• Positive WB detected in: A549 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29603-1-AP targets BICD2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31279

  Product name: Recombinant human BICD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 361-523 aa of BC073970

  Sequence: GLLATLQDTQKQLEHTRGSLSEQQEKVTRLTENLSALRRLQASKERQTALDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKVSLLEKASRQDRELLARLEKELKKVSDVAGETQGSLSVA

• Full Name: bicaudal D homolog 2 (Drosophila)
• Calculated Molecular Weight: 855 aa, 97 kDa
• Observed Molecular Weight: 93-96 kDa
• GenBank Accession Number: BC073970
• Gene Symbol: BICD2
• Gene ID (NCBI): 23299
• RRID: AB_2935474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TD16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein bicaudal D homolog 2 (BICD2) acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:25962623).