SURVIVIN-Specific Polyclonal antibody

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  WB analysis of HeLa using 19119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19119-1-AP (SURVIVIN-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 19119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19119-1-AP (SURVIVIN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 19119-1-AP

  Immunofluorescent analysis of HeLa cells using 19119-1-AP (SURVIVIN-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SURVIVIN-Specific Polyclonal Antibody for ELISA

Cat No. 19119-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

ELISA

Synonyms

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN

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Product Information

19119-1-AP targets SURVIVIN-Specific in ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: baculoviral IAP repeat-containing 5
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_001168
• Gene Symbol: Survivin
• Gene ID (NCBI): 332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15392
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIRC5, also named as API4 and IAP4, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Isoforms 2 and 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. BIRC5 may play a role in neoplasia and counteract a default induction of apoptosis in G2/M phase. The antibody can recognize all these isoforms of BIRC5.