BIRC8 Polyclonal antibody

BIRC8 Polyclonal Antibody for WB, ELISA

Cat No. 20144-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Baculoviral IAP repeat-containing 8, hILP2, IAP like protein 2, ILP 2, ILP2

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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WB analysis of mouse brain using 20144-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20144-1-AP (BIRC8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 20144-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20144-1-AP (BIRC8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for BIRC8 in ?

Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive WB detected in

mouse brain tissue, human brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20144-1-AP targets BIRC8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13658 Product name: Recombinant human BIRC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC039318 Sequence: MTGYEARLITFGTWMYSVNKEQLARAGFYAIGQEDKVQCFHCGGGLANWKPKEDPWEQHAKWYPGCKYLLEEKGHEYINNIHLTRSLEGALVQTTKKTPSLTKRISDTIFPNPMLQEAIRMGFDFKDVKKIMEERIQTSGSNYKTLGVLVADLVSAQKDTTENELNQTSLQREISPEEPLRRLQEEKLCKICMDRHIAVVFIPCGHLVTCKQCAEAVDRCPMCSMVIDFKQRVFMS Predict reactive species
Full Name	baculoviral IAP repeat-containing 8
Calculated Molecular Weight	338aa,39 kDa; 236aa,27 kDa
Observed Molecular Weight	30-35 kDa
GenBank Accession Number	BC039318
Gene Symbol	BIRC8
Gene ID (NCBI)	112401
RRID	AB_10732683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6PIA0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The BIRC8 Polyclonal antibody (Cat# 20144-1-AP) has 1 citation. It appears in Oncotarget (2016), where it was used in Western blot to study the role of ARHGEF3 in promoting nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis. The associated research field is oncology, specifically tumor biology and apoptosis in head and neck cancers.

Species	Application	Title
human	WB	Oncotarget The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis. Authors - Tian-Hao Liu View Article